By Hongtao Guan, Endre Kiss-Toth (auth.), Meike Werther, Harald Seitz (eds.)
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In addition, we believe that developing novel assays which do not necessarily require a major capital investment is of paramount importance. Examples of such approaches include modifications of the classical Y2H, the MAPPIT system or functional expression cloning. These enable access to cutting edge biology research for the wide scientific community, thus ensuring that knowledge is both shared and accumulated globally. 20 H. Guan · E. Kiss-Toth References 1. Alberts B (1998) The cell as a collection of protein machines: preparing the next generation of molecular biologists.
4 Integrative Systems Analysis In the foregoing sections, we have briefly summarized excitation-contraction coupling and its regulatory mechanism as well as mathematical models for cardiac systems biology. We find that the intracellular Ca2+ dynamics are regulated by complex mechanisms of a variety of ion channels, transporters, and exchangers. Hence, to investigate the regulatory mechanisms and hidden properties of intracellular Ca2+ dynamics of the cardiac myocytes, a “system-level integrative analysis” is required.
3 Quantification for Functional Analysis . . . Mathematical Modeling . . . . . . . Sensitivity Analysis in Systems Biology . . . Quantification of Functional Regulatory Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Parameter Sensitivity Analysis of Ca2+ Regulatory Mechanisms Sarcoplasmic Reticulum Calcium Pump (SERCA) . . . . . Sarcolemmal l-Type Calcium Channels (LTCC) . . . . . Sarcoplasmic Reticulum Calcium Release Channels (RyR) .