By Frank Stahl, Bernd Hitzmann, Kai Mutz (auth.), Wei Shou Hu, An-Ping Zeng (eds.)
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Example text
Specifically, the use of microarrays can accomplish the following goals: • Compare the time course of a sensor signal with the actual genomic response. • Identify genes that respond earlier or more specifically to toxins. • Identify gene induction patterns that can identify one toxin versus another (which can in turn be incorporated into multichannel sensors). In the last few years, functional transcriptomics has been advanced by both microarray technology and genome sequencing. Certainly microarray technology has achieved its technical limits and is more and more complemented by 22 F.
Com. 16 F. Stahl et al. excitation from each cluster. The identity of the first base is recordable. The elimination of the 30 blocked terminus and the dyes follows. Within each new cycle, the DNA chain is elongated and more images are recorded for analysis. Here the reading frame is tenfold smaller (30 nt) than with common pyrosequencing. The whole system has is closely related to the method from Helicos BioScience. Applications of this method are sold by both Illumina, Inc. (San Diego, CA, USA) as well as Solexa, Inc.
In the so-called sandwich format, capture antibodies are immobilized on the microarray surface and the binding of the corresponding protein is detected via labelled detection antibodies (Fig. 6). Therefore, for each target protein, two antibodies binding to different epitopes of the target are required. Sandwich-based microarrays avoid the difficulties associated with labelling reactions and exhibit high sensitivity [57]. Moreover sandwich assays are known to be highly specific, since the target must be recognized by two different antibodies.