By Handel, Tracy M
Chemokines, the newest quantity within the Methods in Enzymology sequence, keeps the legacy of this most efficient serial with caliber chapters authored via leaders within the box. This quantity covers examine equipment in chemokines, and contains sections on such subject matters as chemokine detection utilizing receptors, monitoring mobile responses to chemokines, attractiveness of GAG-bound chemokines, and the construction of chemokine receptor complexes for structural and biophysical studies.
- Continues the legacy of this preferable serial with caliber chapters authored through leaders within the field
- Covers learn tools in chemokines
- Contains sections on such themes as chemokine detection utilizing receptors, monitoring mobile responses to chemokine, reputation of GAG-bound chemokines, and the creation of chemokine receptor complexes for structural and biophysical studies
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Originally published in Hockemeyer et al. (2014). consequently generated within a very small volume places some stress on the cells. Thus, it is very important to carefully regulate the flow rate. , 2012). , 2012). Another disadvantage of microfluidic devices is the limited number of samples that may be assayed at the same time due to the complexity of the assay. Additional modifications are needed to allow the testing multiple Microfluidic Devices 39 Figure 8 Spheroid sprouting in response to addition of CXCL12.
Yang, H. , & Li, C. M. (2010). Optical detection of single cell lactate release for cancer metabolic analysis. Analytical Chemistry, 82(12), 5082–5087. doi. 1021/ac100074n. , et al. (2012). Frequency-dependent Escherichia coli chemotaxis behavior. Physical Review Letters, 108(12), 128101. , Dunn, G. , & Brown, A. F. (1991). A new direct-viewing chemotaxis chamber. Journal of Cell Science, 99(Pt. 4), 769–775. Zigmond, S. H. (1977). Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors.
Dunn, G. , & Brown, A. F. (1991). A new direct-viewing chemotaxis chamber. Journal of Cell Science, 99(Pt. 4), 769–775. Zigmond, S. H. (1977). Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. The Journal of Cell Biology, 75(2 Pt. 1), 606–616. ch Contents 1. 3 Applying Phage Display Technology to Chemokine Receptors 2. 2 Selection of Libraries on Cells 3. 1 The Atypical Chemokine Receptor DARC (ACKR1) 4. 4 A Prototypic Inhibitor of CX3CR1, the Fractalkine/CX3CL1 Receptor References 48 48 49 50 50 52 60 65 65 68 68 68 69 69 70 Abstract Phage display technology, which allows extremely rare ligands to be selected from libraries of variants according to user-defined selection criteria, has made a huge impact on the life sciences.