By Shouichi Yoshida, Douglas A. Forno, James Cock
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55 CHAPTER 14. Determination of 14C-labeled starch in plant tissue. Equipment Packard Tri-Carb Model 314 EX Liquid Scintillation spectrometer and appropriate glass vials, centrifuge, 50-ml centrifuge tubes, mortar and pestle. Sample preparation Reagents 1) Sodium chloride, 20 percent. Dissolve 200 g NaCl in 1 liter distilled water. 2) Iodine-potassium iodide reagent. 5 g potassium iodide with 150 ml of distilled water in a mortar and grind with a pestle. Dilute to 250 ml with distilled water and filter through a Whatman No.
0 Glucose stock solutions and standards should be prepared daily and kept Procedure Put 5 ml of starch extract into a 50-ml volumetric flask and make up to volume with distilled water. Put 5 ml of this diluted starch extract into a Pyrex test tube (see note), and then put this tube and the tubes containing the standards into an ice bath. To each tube, slowly add 10 ml of the anthrone reagent, allowing the reagent to run down the side of the test tube. Stir slowly with a glass rod. 5 minutes. Then immediately cool in ice.
1968. An extraction procedure of plant materials for the rapid determination of Mn, Cu, Zn, and Mg by the Atomic absorption analysis. J. Sci. Soil Manure, Japan 39:489-490. 33 Leyton, L. 1954. Phosphate interference in Flame photometric determination of calcium. Analyst. 79:497-500. Perkin-Elmer Corp. 1971. Analytical methods for atomic absorption spectrophotometry. Norwalk, Connecticut, U. S. A. 34 CHAPTER 7. Dithizone test for heavy metals in solution. Equipment Four-liter and 125-ml Pyrex, Glass-stoppered, separating funnels and racks for holding them, 1-liter Pyrex bottle, filter funnel and Whatman filter paper No.