By Michael C. Flickinger
Biotechnology represents an immense region of analysis concentration, and plenty of universities are constructing educational courses within the box. This consultant to biomanufacturing includes conscientiously chosen articles from Wiley's Encyclopedia of commercial Biotechnology, Bioprocess, Bioseparation, and phone Technology in addition to new articles (80 in all,) and contours an identical breadth and caliber of assurance and readability of presentation present in the unique. For teachers, complex scholars, and people concerned about regulatory compliance, this two-volume table reference deals an obtainable and complete source.
Read Online or Download Upstream Industrial Biotechnology, Volume 1: Expression Systems & Process Development & Volume 2: Equipment, Process Design, Sensing, Control, and cGMP Operations PDF
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Extra resources for Upstream Industrial Biotechnology, Volume 1: Expression Systems & Process Development & Volume 2: Equipment, Process Design, Sensing, Control, and cGMP Operations
Sample text
50. Farid SS, Washbrook J, Titchener-Hooker NJ. Biotechnol Prog 2005; 21: 486–497. 51. Singh V. Cytotechnology 1999; 30: 149–158. 52. Hyclone web information. php. Accessed 2007 Aug 21. 53. Xcellerex Inc. Web information. xcellerex. pdf. Accessed 2007 Aug 21. 54. Lim AC, Washbrook J, Titchener-Hooker NJ, Farid SS. Biotechnol Bioeng 2006; 93: 687–697. 55. Xie L, Zhou W. In: Ozturk SS, Hu W-S, editors. Cell culture technology for pharmaceutical and cell-based therapies. Florida: Taylor & Francis; 2006.
Cell culture technology for pharmaceutical and cell-based therapies. Florida: Taylor & Francis; 2006. p 155–224. 37. Lubiniecki A, Arathoon R, Polastri G, Thomas J, Wiebe M, Garnick R, Jones A, van Reis R, Builder S. In: Spier RE, Griffiths JB, Stephenne J, Crooy PJ, editors. Advances in animal cell biology and technology for bioprocesses. London: Butterworth; 1989. p 442–449. 38. Adamson R. Ann Hematol 1994; 68: S9–S14. 39. Deo YM, Mahadevan MD, Fuchs R. Biotechnol Prog 1996; 12: 57–64. 40. Ray NG, Rivera R, Gupta R, Mueller D.
The BaculoDirect linear DNA is mixed with a donor Gateway vector in a 1 h enzymatic reaction and replacement of both HSV1-tk and β-galactosidase with the foreign gene is achieved. Removal of HSV1-tk causes the virus to be insensitive to ganciclovir after the genome is used to transfect insect cells (20). Removal of β-galactosidase provides another level of selection. The advantage of this system is that baculovirus genome manipulation is done in vitro without the need to use bacterial cells. Plaque assay purification of virus after transfection of insect cells is also not required.