By Malcolm J. Hawkesford (auth.), Malcolm J. Hawkesford, Peter Buchner (eds.)
Adverse environmental elements can impose tension on crops and impact the expression of the whole genetic strength for development and replica. the potential of crops to advance plastic reaction reactions, to conform to environmental tension events, is exclusive within the organic international. A target of the learn defined during this quantity is to extend crop productiveness, specific in areas the place the surroundings imposes tension. An figuring out of the foundations fascinated by plant version to environmental tension will let optimisation of practices to enhance agronomic construction and minimise harmful environmental impression. the purpose of this quantity is to hyperlink the swiftly advancing and more and more professional box of molecular biology with plant body structure on the atmosphere point. The booklet contains chapters inquisitive about a few precept equipment and a sequence of up to date assessment chapters on plant edition to numerous particular stresses. The utilisation of newly to be had genome info is emphasized. Of specific significance is the need to focus on the present capability of such techniques, and the way various disciplines can engage and supplement each other. The publication is aimed toward either the expert and the complex student.
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1999. Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. Biotech. 17, 287-291. , van Montague, M. P. 1991. In vivo random B-glucuronidase gene fusions in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 88, 5212-5216. A. L. 1991. Production of sodium-chloridetolerant Brassica juncea plants by in vitro selection at the somatic embryo level. Theor. Appl. Genet 83, 233-237. J. R. 1999. The sfr6 mutation in Arabidopsis suppresses low-temperature induction of genes dependent on the CRT/ORE sequence motif.
To obtain gene silencing most experiments typically use a complete or partial eDNA clone placed downstream of a strong promoter in sense or anti-sense orientation. , enzymes in anthocyanin colouration (Meyer and Saedler, 1996). , 1998). By the transformation of specific gene constructs that confer aberrant or double stranded RNA quite predictable silencing of homologous genes in the genome is likely. This approach of HDGS is also applicable to create mutations for multigene families, for example many of the stress-induced transcripts, where clues to their biological role may be obtained.
Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Mol. Gen. Genet. 233,449-461. C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant Bioi. 2, 109-113. Bechtold, N. and Pelletier, G. 1998. In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol. Bioi. 82, 259-266. S. 1982. Plant productivity and environment. Science 218, 443-448. , Wykoff, D. and Mitchell, D.