By R.H. Templer, R.J. Leatherbarrow
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Extra resources for Biophysical Chemistry: Membrane and Proteins (Biotechnology Intelligence Unit, 283)
Example text
Contact, or ‘tapping’, mode consists of rapidly oscillating (mechanically or magnetically) the cantilever in the z direction whilst slowly lowering it towards the surface and scanning. When the cantilever comes close to the surface, the amplitude of the oscillations is dampened and the surface can be detected. This mode is much less damaging to samples”. Finally, in force-distance measurements, the cantilever is slowly lowered towards the surface and its deflection is constantly recorded. This yields a curve showing the Biophysical Chemistry: Membranes mid Prorrim Figure 1 Equipment setup of the bio-AFM (LHS),A schematic illustration of the AFM operation.
In comparison, the resolution of confocal laser scanning microscopy is limited by the wavelength of the light used and is significantly lower than that of scanning electron microscopy. However, as resolution is proportional to the deformation caused by the force used for imaging, contact imaging with higher forces can reveal relatively 'hard' intracellular structures in soft, unfixed cells (Fig. 2B). Operation of the AFM in non- 36 Biophysical Chemistry: Membranes and Proteins contact mode can enhance the resolution where it can reach the order of a few tens of nanometers7’.
Gas phase MRCI parameters, comprising 6 charges fitted to reproduce the molecular dipole and quadrupole, show an improved correlation at 220 nm. The CASSCF parameters derived from condensed phase studies are the first to show moderate correlation at 190 nm and 208 nm. This is initially surprising since the CASSCF wave function is inferior to the MRCI wave function, containing no description of dynamic correlation. However, this improved agreement arises from an improved orientation of the &bn* transition dipole moment.