By Gülsen Ökten (auth.), Kursad Turksen (eds.)
This quantity will disguise a sequence of stories on stem cells together with grownup and embryonic stem cells. audio system have been invited to give those talks through the Stem mobile Symposia in fall of 2010, in Samsun, Turkey. detailed element of this quantity is that it brings a multidisciplinary point of stem cells extracted from a symposium.
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In addition, some of these PDGFRa+/YFP + cells were MSCs. 5 embryos derive from both a neural/NC pathway and a nonneural pathway. Takashima et al. (2007) then analyzed the contribution of these neural/NC-derived MSCs to the postnatal BM. 39%, respectively). 35% for sox1-cre/yfp and P0-cre/yfp mice at P28 (post-natal day 28), respectively]. Finally, the frequency of PDGFRa + MSCs derived from YFP + compared with YFP − populations was nearly the same. Thus, in neonatal and adult bone preparations, MSCs were found within the PDGFRa + population, but most of them derived from a nonneural/NC pathway.
1995; Arthur et al. 2008; Horwitz et al. 2002). However, they are scarce in tissues and the necessity of in vitro proliferation in culture media is the major disadvantage of using MSCs in basic science researches and clinical settings. In vitro proliferation in culture media leads to changes in phenotypic, immunological and other biological properties of these cells due to exposure to various stimulators and factors during passage in culture. There is a risk of cell aging, cytogenetic disruption and although low, malignant transformation when cells are proliferated by passaging in culture media.
1999; Pittenger et al. 1999; Choi et al. 2005), provided a basis for the proposition of a model in which pericytes are stem cells throughout the vasculature, contributing to the replenishment of lost cells under physiological conditions and possibly assuming a more active role during tissue injury (da Silva Meirelles et al. 2006). In further support of this concept, intact pericytes in their tissue of origin natively express the MSC markers CD44, CD90, CD73, CD105, and CD146 (Crisan et al. 2008; Schwab and Gargett 2007).