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1999. Improving plant drought, salt and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nat. Biotech. 17, 287-291. , van Montague, M. P. 1991. In vivo random B-glucuronidase gene fusions in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 88, 5212-5216. A. L. 1991. Production of sodium-chloridetolerant Brassica juncea plants by in vitro selection at the somatic embryo level. Theor. Appl. Genet 83, 233-237. J. R. 1999. The sfr6 mutation in Arabidopsis suppresses low-temperature induction of genes dependent on the CRT/ORE sequence motif.
To obtain gene silencing most experiments typically use a complete or partial eDNA clone placed downstream of a strong promoter in sense or anti-sense orientation. , enzymes in anthocyanin colouration (Meyer and Saedler, 1996). , 1998). By the transformation of specific gene constructs that confer aberrant or double stranded RNA quite predictable silencing of homologous genes in the genome is likely. This approach of HDGS is also applicable to create mutations for multigene families, for example many of the stress-induced transcripts, where clues to their biological role may be obtained.
Development of an efficient two-element transposon tagging system in Arabidopsis thaliana. Mol. Gen. Genet. 233,449-461. C. 1999. Fast forward genetics based on virus-induced gene silencing. Curr. Opin. Plant Bioi. 2, 109-113. Bechtold, N. and Pelletier, G. 1998. In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol. Bioi. 82, 259-266. S. 1982. Plant productivity and environment. Science 218, 443-448. , Wykoff, D. and Mitchell, D.