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Extra resources for A brief course in the calculus, by William Cain.
The first is that we use invertebrate preparations in which we can identify specific cells and return to them repeatedly. The second is the great advance made in the techniques of intracellular staining. Once electro physiological measurements have been made, identifiable neurons can be selectively stained for cytoarchitectural studies at both the light and electron microscopic levels. A great deal of useful information can be obtained by simply looking at serial sections or whole mounts of such selectively stained preparations.
Lm. Lm, since (1) in thicker sections the background fluorescence begins to obscure the detail of the finest processes and (2) thinner sections necessitate longer photographic exposure times to bring out the Procion-filled contours. Also, the thinner the sections, the greater the amount of core memory space needed in the later computer-assisted steps of the reconstruction. Photography of the sections is done using a Zeiss universal photomicroscope. Sections are illuminated with ultraviolet light passed through a BG 12 exciter filter (peak transmission at 400 nm) and spectrally sharpened with an FITC 490-nm interference filter.
The equations which describe such leaky cables use the length and diameter of the process as the principal anatomical variables and the axoplasmic and membrane resistance as the main electrical ones. The most difficult values to obtain experimentally are the location of the synapses on the processes (the Computer Reconstruction of Invertebrate Nerve Cells 43 actual origin of the potential) and the location on the processes from which electrical measurements happen to be made. We had hoped that double injections of functionally related cells would reveal points of contact that could reasonably be inferred to be the synaptic loci.